TLC e bonolo mekhoa and analysis rarahaneng

TLC ke moeta-pele ea ka litaba tsa and analysis ditirisanommogo le seka-ditirisanommogo tsa meriana rarahaneng, tsa tlhaho, ea biomedical, theknoloji, lik'hemik'hale le lintho tse ling tse ngata. Thin lera chromatography ke e boetse e le mokhoa ho fetisisa ho fihlella ya and analysis boima ba hoo e batlang e litlelase kaofela dintho.

Lewa lena la karohano le ho hlahloba tsamaiso ea selotlolo motsoako ba ho pharaletseng sebelisoa ka dilaboratiriamong ea khethehileng fapaneng haholo le tataiso: bohloeki-epidemiological, a diphoofolo, agrochemical, lipatlisiso, forensiki, 'me bakeng sa ho liteishene tsa tšireletso ea dimela, Setsi sa gore maemo le Bureau ea forensiki Medicine.

Melemo e ka sehloohong ea le basesaane-lera chromatography, e thehiloeng tshisinyo eluent (analyte solvent) ka sorbent lera ka e le bitsoa mabotho capillary ke tsela e bonolo le easiness ea ho chromatographic, tekanyo e phahameng ea sebetsa ka ho nepahetseng ba and analysis le theko e batlang e tlaase ea thepa e hlokahalang.

TLC, ho ba le lengolo la phahameng tsa nahanela (ka tlaase bonoa monyako) le selectivity, o dumella ho bona hore 10-20 ug dintho ho fihlela ho 7%, eo e phahameng haholo. Thin lera chromatography ka ba adsorption le kabo mefuta. Ka ho fetisisa ho pharaletseng sebediswa ke khetho ea pele.

Motheo oa mokhoa o larileng ka TLC solvent ntho susumeletsa ka sorbent bethe le kabo tlhaloganya sebaka ea likarolo tse fapa-fapaneng ho latela litšobotsi tse bona motho le thepa. 'nete ea hore limolek'hule tsa sebopeho fapaneng le sebōpeho sa adsorbed le (beha) holim'a poleiti tiileng chromatographic tsela e fapaneng. Ka hona, e rarahaneng tsamaiso ea selotlolo motsoako ba butle-butle a arohana ka dikarolo ka bomong.

Hatela ka lebelo fapaneng hammoho le sorbent bethe, motho eo e se e arohane, metsoako e theha e chromatogram. E mong le e 'mala letheba tšoana le k'hemik'hale e itseng. Colorless ntho le mahlaseli khanya tlas'a tšusumetso ea ba eo plurality tsa metsoako qala ho fluoresce, kapa tlas'a khethehileng kalafo colouring reagent.

Har'a morao-rao mekhoa katisediwa GLC ikhethang. Mokhoa ona e tšoauoa ka karohano le and analysis sebelisa, e le sorbing Moemeli ea khethehileng nonvolatile mokelikeli, ao ho 'ona ho phofshoana e lintho tse bonahalang ceramic e wetted. 'Me e le mahareng khanna le sebelisitsoeng mona le fapaneng likhase inert. Mokhoa ona ke atlehang ka ho fetisisa bakeng sa ho ithuta sa metsoako e rarahaneng hore na tse makholo dikarolo fapaneng.

E mong ea kajeno mokhoa bakeng sa batlisisa le ho hlophisoa ha dintho rarahaneng e mokelikeli chromatography. The tšobotsi e ka sehloohong ea mokhoa ona e na le ka karohano ea likarolo motho ka ho sebelisa ho se tšoane ha kabo bona pakeng tsa litlhaku (eluent) le e tsitsitseng (adsorbent) mekhahlelo e. Mokhoa ona e hangata ka ho fetisisa sebelisoa ka ho hlahloba metsoako e sa tšoaneng manyolo (additives lijo, chefo e bolaeang likokoanyana, tse fapa-fapaneng chefo, joalo-joalo). Hape, jwalo mokhoa chromatographic e sebelisoa ho ithuta ho laola maikutlo boleng ba lihlahisoa meriana, boikemisetso ba steroid ka maro a likokoana-hloko, 'me tse ling tse and analysis amino acid e.