Recombinant erythropoietin. Recombinant erythropoietin batho

Erythropoietin (EPO) - glycoprotein hormona hore laola lebelo la erythropoiesis batho. The komponeng joale e tsa maiketsetso haholo-holo ka ho phenya liphio, e leng palo e nyenyane (ka liphesente tse 10) e hlahisoa sebete. Hormone erythropoietin activates karohano le khethollo ea ba progenitors erythroid. Maemo a e se nang sesosa hormona ka lero la mali mali a batho ka bophelo bo botle fapana ka hare ho mefuta e fapaneng ya mefuta e 'me ke inversely tekanyo e lekanang ya ho haemoglobin mahloriso le tekanyo ea ho oxygenation tsa lisele. Mosebetsi oa ka ho itšehla thajana le ho hlahisa li-hormone ena e le hore a moriana na le thuso haholo.

Sebopeho le boleng li-hormone

The molek'hule e na le amino acid hormona. Ka khaello ea e se nang sesosa erythropoietin e bile ea fokotseha bohale boemo ba haemoglobin le lisele tse khubelu tsa mali, e tsoelang pele eo ho thoeng ke eritropoetindefitsitnaya khaello ea mali. o ile a lula ha khoneha ka lebaka la ho haella ha meriana loketseng ho tse fihlela morao tjena, jwalo khalemelo lithethefatsi ba khaello ea mali. Kajeno, ka haelloa holimo hormone ka 'mele oa motho, lingaka ho laela recombinant erythropoietin. Ho lokisetsa fumaneha lisele tse phoofolo eo hlahiswa phatsa ea lefutso khoutu ya EPO batho. Recombinant erythropoietin batho ka amino acid e le k'habohaedreite sebopeho ke le tsoanang ho hormone tsa tlhaho, ho eketsa palo ea lisele tse khubelu tsa mali, reticulocytes, activates le biosynthesis ea haemoglobin ka lisele tsa tsona li. Mosebetsi likokoana-hloko tsa lintho tse bonahalang sephetho le ho na fapaneng le hormone e se nang sesosa. Recombinant erythropoietin ha bontša litla cytotoxic le ha e ame ho leucopoiesis. Bo-rasaense ba bolela hore EPO le sebedisana ka le receptors itseng eritropoetinchuvstvitelnymi, tse teng holim'a seleng.

A tshepetso ho hloekisa recombinant erythropoietin batho

Recombinant batho EPO - ke e 'ngoe ea liprotheine haholo le ngata e hlahisoang ke lik'hamphani tse ngata tsa likokoana-hloko le meriana ho pota lefatše bakeng sa phekolo ea lithethefatsi. The komponeng joale e tsa maiketsetso lisele tse ovary Chinese mmutlanyana (Cho) ke dithekeniki recombinant DNA. E mong polypeptide ketane recombinant EPO e na 165 amino acid, e balwa limolek'hule boima ba 24.000 Yeah, 'me ba ile ba hlokomela limolek'hule Boima ba glycosylated protheine - 30 400 Da. Branch erythropoietin ho tloha litšila e etsoang ke Ion-phapanyetsano le amana chromatography. Batho recombinant EPO e na le hloekileng 98%.

Recombinant erythropoietin le analogs lona

Hore a susumetse erythropoiesis thulaganyou e lingaka li sebelisa lithethefatsi tse sa tšoaneng:

• "Aranesp";
• "Aeprin";
• "Epobiokrin";
• "Bioein";
• "Vepoks";
• "Binokrit";
• "Epokrin";
• "Gemax";
• "Epogen";
• "Eprex";
• "Epovitan";
• "Epomaks";
• "Giperkrit";
• "Eralfon";
• "Eritrea";
• "Recormon";
• "Epostim";
• "Epozino";
• "Epoetin Beta".

Pele u nka sebaka ya recombinant erythropoietin analogues, lokela ho a bale ngaka.

ho bonahala

lingaka tsa bongaka hangata ba ho sebetsana le eritropoetindefitsitnymi khaello ea mali (EDA). sehlopha sena se akarelletsa ho pathologies latelang:

• khaello ea mali ea neoplasms bolaeang;
• mathoasong a khaello ea mali ea toddlers prematurity (pele libeke tse 34 emara) ka boima ba 'mele ea 750 ho 1500 g,;
• nephrogenic khaello ea mali;
• Khaello ea mali ka mafu a sa foleng (hepatitis C, e ruruhisang ramatiki, tšoaetso ea HIV, le mafu alimentary kanale).

A tšobotsi e khethehileng ea ka holimo-khaello khaello ea mali ke 'nete ea hore ha ba tšoaroa ka litokisetso tšepe. Ho fihlela morao tjena, feela phekolo e atlehang ke tšeloa. Ka lebaka la 'nete ea hore mofuta ona oa phekolo o na le palo e khōlō ea litla-morao le kotsing e khōlō ea phetiso ya likokoana-hloko mali-jere tsa mafu a kotsi a tšoaetsanoang (HIV, lefu la sebete livaerase, joalo-joalo), mokhoa ona ka moriana morao-rao e hoo e ka bang mohla o ile a sebelisa. Recombinant erythropoietin batho thusa ho ntlafatsa boleng ba bophelo ba bakuli le phumano ya "eritropoetindefitsitnaya khaello ea mali." Biosynthesis le selelekela ea recombinant EPO batho bula mehla e mecha ea ho phekola a phokolo ea mali eritropoetinzavisimyh.

Contraindications

Lingaka li u se ke ua ho kgothaletsa ho sebelisa recombinant erythropoietin (pukwanana lemosa ka eona) ka ho ba teng ha pathologies latelang:

• thromboembolism;
• hypersensitivity ho moriana;
• khoneha ya anticoagulant phekolo ka tsela e atlehang
• isoa bokong setorouku kapa myocardial infarction;
• tsitsang angina;
• sa laoleheng kgatelelo ya madi;
• nako ea ho ba le bana le ho nyantsha;
• refractory kgatelelo ya madi.

dosing Melao

The litekanyetso li behoa 'me nako ea phekolo sekemeng ba thehilwe motheo tieo motho, ho itšetlehile ka ho tiea la khaello ea mali, boemo kakaretso oa mamello le mofuta wa bolwetse le. moriana o ne a rerile hore tšebeliso parenteral. tekanyetso ea pele ka fapana ka mefuta e fapaneng ho tloha 50 ho ea ho tse 150 iappa / lik'hilograma. lethal dose lokela ho fetola ho itšetlehile ka lilemo tsa ho mokuli. Recombinant erythropoietin hangata laolwa makhetlo a 3 ka beke. Ha overdose e se e ntse e eketseha litla-morao. Phello e ba ho feta kapa ka tlaase ho bonahalang ka mor'a libeke tse 2-3 ya kopo.

setlamorao

O e behiloeng recombinant erythropoietin batho? Litaelo tsa Sebelisa ha kgetholla ntshetsopeleng ya litla-morao. Ba:

• myalgia;
• ho tsekela;
• hyperthermia;
• otsela;
• ho hlatsa;
• hlooho;
• letšollo;
• arthralgia;
• kgatelelo ya madi;
• bohloko sefubeng;
• thrombocytosis;
• tachycardia;
• hypertension bothata;
• tsitsipana;
• steatosis;
• ho tsosa boemo ba AST, Alt maling;
• erythrocyte kokoana-hloko li-cell aplasia;
• eczema;
• angioedema;
• letlalo lekhopho le ho hlohlona;
• asthenia;
• hives, redness le tukang ka sebaka sa tsamaiso ea;
• hyperkalemia;
• phokotso ea protheine mahloriso ka lero la mali ferritin;
• hyperphosphatemia.

Erythropoiesis tse susumetsang ka lipapali

Morao tjena, sebelisoa hangata lithethefatsi "Epovitan" (recombinant batho erythropoietin). The sesebelisoa joale e atisa ho sebelisoa ka lipapali (mabelo, bodybuilding, ho sesa, biathlon). O ile a re ho lokisetsa activates le biosynthesis tsa lisele tse khubelu tsa mali, e leng ka lehlakoreng le e etsa hore ho eketseha ha diteng tsa oksijene ka unit trust bophahamo ba modumo ea mali 'me, ka lebaka leo, ho eketseha ka bokgoni mali oksijene' me O ₂ pelehi ho makala 'me lisele tse. mochine ona bohato eketsa ka mamello aerobiki ea moatlelete e. A le phello e tšoanang o ile a re ha ba koetlisa semathi ka Midlands ka, moo sechaba se haellang ho Uena ₂ moeeng ho etsa hore tsoelo-pele ea hypoxia, eo le eena activates le biosynthesis ea e se nang sesosa erythropoietin. EPO litokisetso li sebelisoa hammoho le oa ho thusa insulin, khōlo ea li-hormone (albagbogg, somatotropin) le stanazolol.

Ka tsela e feteletseng, se laole tšebeliso ea "Erythropoietin" lithethefatsi lipapaling ka etsa hore clots mali hore hangata se isa lefung. Lemoha recombinant EPO e thata haholo, hobane a maiketsetso a metsoako sebopeho ke le tsoanang ho analoge lona fisioloji, kahoo biosoedinenie ena ntse molao sebelisoa ka lipapali phahameng tshebetso ka doping.